The second regional rankings, released Thursday, May 5, 2016.
Teams in bold have won their conference automatic bid. Teams that will need an at-large bid are in italics. Five unranked, Castleton, Huntingdon, Pacific Lutheran, Salve Regina, and Suffolk have already earned playoff bids.
| Central Region | ||||
| 1 | North Central (Ill.) | 26-6-0 ( 0.813) | 26-6-0 ( 0.813) | |
| 2 | Wartburg | 28-8-0 ( 0.778) | 28-8-0 ( 0.778) | |
| 3 | Buena Vista | 26-8-0 ( 0.765) | 26-8-0 ( 0.765) | |
| 4 | Washington U. | 27-16-0 ( 0.628) | 28-16-0 ( 0.636) | |
| 5 | Augustana | 23-12-0 ( 0.657) | 23-12-0 ( 0.657) | |
| 6 | Webster | 22-12-0 ( 0.647) | 24-13-0 ( 0.649) | |
| Mid-Atlantic Region | ||||
| 1 | Misericordia | 30-10-0 ( 0.750) | 30-10-0 ( 0.750) | |
| 2 | TCNJ | 28-8-0 ( 0.778) | 28-8-0 ( 0.778) | |
| 3 | Keystone | 27-8-0 ( 0.771) | 27-8-0 ( 0.771) | |
| 4 | Johns Hopkins | 26-11-2 ( 0.692) | 26-11-2 ( 0.692) | |
| 5 | Ramapo | 29-9-1 ( 0.756) | 29-9-1 ( 0.756) | |
| 6 | Kean | 24-14-0 ( 0.632) | 24-14-0 ( 0.632) | |
| 7 | Haverford | 24-12-0 ( 0.667) | 24-12-0 ( 0.667) | |
| 8 | Rutgers-Camden | 23-14-0 ( 0.622) | 23-14-0 ( 0.622) | |
| Mideast Region | ||||
| 1 | La Roche | 30-8-0 ( 0.789) | 30-8-0 ( 0.789) | |
| 2 | Marietta | 22-13-0 ( 0.629) | 22-13-0 ( 0.629) | |
| 3 | Ohio Wesleyan | 25-11-0 ( 0.694) | 25-11-0 ( 0.694) | |
| 4 | Case Western Reserve | 26-13-0 ( 0.667) | 26-13-0 ( 0.667) | |
| 5 | Wooster | 26-9-0 ( 0.743) | 26-9-0 ( 0.743) | |
| 6 | Rose-Hulman | 23-9-0 ( 0.719) | 23-9-0 ( 0.719) | |
| 7 | Otterbein | 24-7-0 ( 0.774) | 25-7-0 ( 0.781) | |
| 8 | Adrian | 23-8-0 ( 0.742) | 24-9-0 ( 0.727) | |
| 9 | Denison | 22-11-0 ( 0.667) | 22-11-0 ( 0.667) | |
| Midwest Region | ||||
| 1 | UW-La Crosse | 26-7-0 ( 0.788) | 26-7-0 ( 0.788) | |
| 2 | Concordia-Chicago | 28-9-0 ( 0.757) | 28-9-0 ( 0.757) | |
| 3 | UW-Whitewater | 22-9-0 ( 0.710) | 22-9-0 ( 0.710) | |
| 4 | St. Thomas | 23-9-0 ( 0.719) | 23-9-0 ( 0.719) | |
| 5 | St. Mary's (Minn.) | 22-11-1 ( 0.662) | 22-12-1 ( 0.643) | |
| 6 | St. John's | 25-9-0 ( 0.735) | 25-10-0 ( 0.714) | |
| New England Region | ||||
| 1 | Southern Maine | 25-11-0 ( 0.694) | 25-11-0 ( 0.694) | |
| 2 | Wheaton (Mass.) | 27-11-0 ( 0.711) | 27-11-0 ( 0.711) | |
| 3 | Western New England | 28-9-0 ( 0.757) | 28-9-0 ( 0.757) | |
| 4 | Amherst | 21-10-0 ( 0.677) | 21-10-0 ( 0.677) | |
| 5 | Mass-Boston | 24-12-0 ( 0.667) | 24-12-0 ( 0.667) | |
| 6 | Endicott |
25-13-0 ( 0.658) | 25-13-0 ( 0.658) | |
| 7 | MIT | 20-13-0 ( 0.606) | 20-13-0 ( 0.606) | |
| 8 | Tufts | 25-6-0 ( 0.806) | 26-6-0 ( 0.813) | |
| 9 | Mitchell | 27-9-0 ( 0.750) | 27-9-0 ( 0.750) | |
| 10 | St. Joseph's (Maine) | 22-11-0 ( 0.667) | 22-11-0 ( 0.667) | |
| New York Region | ||||
| 1 | Cortland | 34-5-0 ( 0.872) | 34-5-0 ( 0.872) | |
| 2 | Oswego State | 28-7-0 ( 0.800) | 29-7-0 ( 0.806) | |
| 3 | St. John Fisher | 27-8-0 ( 0.771) | 27-8-0 ( 0.771) | |
| 4 | Stevens | 24-13-0 ( 0.649) | 24-13-0 ( 0.649) | |
| 5 | Ithaca | 20-14-0 ( 0.588) | 20-14-0 ( 0.588) | |
| 6 | Brockport | 22-16-0 ( 0.579) | 22-16-0 ( 0.579) | |
| South Region | ||||
| 1 | Emory | 30-9-0 ( 0.769) | 30-10-0 ( 0.750) | |
| 2 | Birmingham-Southern | 35-11-0 ( 0.761) | 37-11-0 ( 0.771) | |
| 3 | Randolph-Macon | 32-6-1 ( 0.833) | 32-6-1 ( 0.833) | |
| 4 | Salisbury | 25-10-0 ( 0.714) | 25-10-0 ( 0.714) | |
| 5 | Shenandoah | 32-9-0 ( 0.780) | 32-9-0 ( 0.780) | |
| 6 | Frostburg State | 27-10-0 ( 0.730) | 27-10-0 ( 0.730) | |
| West Region | ||||
| 1 | Trinity (Texas) | 33-7-0 ( 0.825) | 35-7-0 ( 0.833) | |
| 2 | Cal Lutheran | 27-10-0 ( 0.730) | 27-11-0 ( 0.711) | |
| 3 | Whitworth | 29-11-0 ( 0.725) | 29-11-0 ( 0.725) | |
| 4 | Concordia (Texas) | 24-13-0 ( 0.649) | 27-13-0 ( 0.675) | |
| 5 | Occidental | 30-7-0 ( 0.811) | 30-8-0 ( 0.789) | |
| 6 | Texas Lutheran | 26-15-0 ( 0.634) | 26-15-0 ( 0.634) |