Regional rankings from Week Two (May 3, 2018)
Schools that have earned an automatic bid are in bold. Schools that are in in Pool B are in italics. La Grange (USA South) and Willamette (NWC) have their conference bids and are not regionally ranked.
| Rank | School | vs. D-III | Overall |
| Central | |||
| 1 | North Central (Ill.) | 22-9 | 22-9 |
| 2 | Wartburg | 21-10 | 21-10 |
| 3 | Webster | 29-7 | 29-7 |
| 4 | Carthage | 26-7 | 27-8 |
| 5 | Washington Univ. |
21-14 | 21-14 |
| 6 | Coe | 18-12 | 19-13 |
| 7 | Augustana | 23-12 | 23-12 |
| 8 | Loras | 21-12 | 21-13 |
| Mid-Atlantic | |||
| 1 | Rowan | 29-8 | 29-8 |
| 2 | The College of New Jersey | 27-7 | 27-7 |
| 3 | Keystone | 26-7 | 26-7 |
| 4 | Ramapo | 29-11 | 29-11 |
| 5 | Alvernia | 28-11 | 28-11 |
| 6 | Arcadia | 25-13 | 25-13 |
| 7 | Misericordia | 27-10 | 27-10 |
| 8 | Rutgers-Camden | 24-13 | 24-13 |
| 9 | Johns Hopkins | 27-11 | 27-11 |
| 10 | DeSales | 25-13 | 25-13 |
| 11 | William Paterson | 23-14 | 23-14 |
| 12 | Penn State Harrisburg | 28-10 | 28-10 |
| Mideast | |||
| 1 | Wooster | 30-5 | 30-5 |
| 2 | Denison | 26-8 | 26-8 |
| 3 | Marietta | 25-11 | 25-11 |
| 4 | Adrian | 28-5 | 28-5 |
| 5 | Otterbein | 26-9 | 26-9 |
| 6 | Case Western Reserve | 25-9 | 25-9 |
| 7 | Washington and Jefferson | 23-10-1 | 23-12-1 |
| 8 | La Roche | 28-7-1 | 28-7-1 |
| 9 | Heidelberg | 23-13 | 23-13 |
| 10 | Franklin | 31-2 | 31-2 |
| 11 | Ohio Northern | 25-11 | 25-11 |
| 12 | Baldwin Wallace | 24-12 | 24-12 |
| 13 | Anderson | 22-13 | 22-14 |
| Midwest | |||
| 1. | Concordia Chicago | 27-8 | 27-9 |
| 2 | UW-Oshkosh | 23-6 | 23-6 |
| 3 | UW-Whitewater | 22-4 | 22-4 |
| 4 | Saint John's (Minnesota) | 24-5 | 24-5 |
| 5 | UW-Stevens Point | 22-8 | 22-8 |
| 6 | UW-La Crosse | 18-9 | 18-9 |
| 7 | Aurora | 22-12 | 22-12 |
| 8 | Bethel (Minnesota) | 23-7 | 23-7 |
| New England | |||
| 1 | Southern Maine | 24-8 | 24-8 |
| 2 | UMass-Boston | 27-7 | 27-7 |
| 3 | Babson | 26-10 | 26-10 |
| 4 | Endicott | 24-12 | 24-12 |
| 5 | Salve Regina | 20-12 | 20-12 |
| 6 | UMass-Dartmouth | 20-12 | 20-12 |
| 7 | MIT | 17-12 | 17-12 |
| 8 | Western New England | 26-11 | 26-11 |
| 9 | Clark | 25-10 | 25-10 |
| 10 | Wheaton (Mass.) | 21-14 | 21-14 |
| 11 | Springfield | 22-13-2 | 23-13-2 |
| 12 | Rhode Island College | 18-12 | 18-12 |
| 13 | Westfield State | 21-14-1 | 21-14-1 |
| 14 | Eastern Connecticut State | 18-12 | 19-12 |
| New York |
|||
| 1 | Cortland | 30-8 | 30-8 |
| 2 | St. John Fisher | 25-10 | 25-10 |
| 3 | Oswego State | 21-11 | 21-11 |
| 4 | Stevens | 23-14 | 23-14 |
| 5 | Ithaca | 20-14 | 20-14 |
| 6 | Union | 25-6 | 25-6 |
| 7 | Farmingdale State | 22-12 | 22-12 |
| 8 | St. Joseph's (L.I.) | 22-14 | 22-14 |
| 9 | New York University | 27-11 | 27-11 |
| South | |||
| 1 | Randolph-Macon | 28-5-1 | 28-5-1 |
| 2 | Christopher Newport | 31-7 | 31-7 |
| 3 | Salisbury | 28-10 | 28-10 |
| 4 | Virginia Wesleyan | 25-12 | 25-12 |
| 5 | Methodist | 30-11 | 32-12 |
| 6 | Berry | 29-13 | 29-13 |
| 7 | Rhodes | 27-13 | 29-14 |
| 8 | Piedmont | 28-14 | 30-14 |
| 9 | Roanoke | 27-9 | 27-9 |
| West | |||
| 1 | Redlands | 31-9 | 31-9 |
| 2 | Chapman | 30-10 | 30-10 |
| 3 | La Verne | 28-12 | 28-12 |
| 4 | Trinity (Texas) | 29-7 | 33-7 |
| 5 | Concordia Texas | 26-13 | 26-13 |
| 6 | Texas-Tyler | 26-14 | 26-14 |
| 7 | Texas Lutheran | 31-9 | 31-9 |
| 8 | Centenary (La.) | 24-14 | 24-14 |